abstract：:To determine if different algal viruses (Phycodnaviridae) share common patterns of seasonal abundance, quantitative PCR methods were developed and applied to monitor the abundances of three different viruses in Lake Ontario, Canada over 13 months. Throughout the year, the abundances of two different phycodnavirus polB...

journal_title：Environmental microbiology

authors： Short SM,Short CM

更新日期：2009-10-01 00:00:00

abstract：:Bacterial degradation of polycyclic aromatic hydrocarbons (PAHs), ubiquitous contaminants from oil and coal, is typically limited by poor accessibility of the contaminant to the bacteria. In order to measure PAH availability in complex systems, we designed a number of diffusion-based assays with a double-tagged bacter...

journal_title：Environmental microbiology

authors： Tecon R,Binggeli O,van der Meer JR

更新日期：2009-09-01 00:00:00

abstract：:Ammonia-oxidizing bacteria (AOB) have long been considered key to the removal of nitrogen in activated sludge bioreactors. Culture-independent molecular analyses have established that AOB lineages in bioreactors are dynamic, but the underlying operational or environmental factors are unclear. Furthermore, the contribu...

journal_title：Environmental microbiology

authors： Wells GF,Park HD,Yeung CH,Eggleston B,Francis CA,Criddle CS

更新日期：2009-09-01 00:00:00

abstract：:Marine sponges constitute major parts of coral reefs and deep-water communities. They often harbour high amounts of phylogenetically and physiologically diverse microbes, which are so far poorly characterized. Many of these sponges regulate their internal oxygen concentration by modulating their ventilation behaviour ...

journal_title：Environmental microbiology

authors： Hoffmann F,Radax R,Woebken D,Holtappels M,Lavik G,Rapp HT,Schläppy ML,Schleper C,Kuypers MM

更新日期：2009-09-01 00:00:00

abstract：:Vibrio cholerae's capacity to cause outbreaks of cholera is linked to its survival and adaptability to changes in aquatic environments. One of the environmental conditions that can vary in V. cholerae's natural aquatic habitats is calcium (Ca(+2)). In this study, we investigated the response of V. cholerae to changes ...

journal_title：Environmental microbiology

authors： Bilecen K,Yildiz FH

更新日期：2009-08-01 00:00:00

abstract：:Actinobacteria are highly abundant in pelagic freshwater habitats and also occur in estuarine environments such as the Baltic Sea. Because of gradients in salinity and other environmental variables estuaries offer natural systems for examining factors that determine Actinobacteria distribution. We studied abundance an...

journal_title：Environmental microbiology

authors： Holmfeldt K,Dziallas C,Titelman J,Pohlmann K,Grossart HP,Riemann L

更新日期：2009-08-01 00:00:00

abstract：:In this paper we present the in silico assessment of the diversity of variable regions of the small subunit ribosomal RNA (SSU rRNA) gene based on an ecosystem-specific curated database, describe a probe design procedure based on two hypervariable regions with minimal redundancy and test the potential of such probe de...

journal_title：Environmental microbiology

authors： Rajilić-Stojanović M,Heilig HG,Molenaar D,Kajander K,Surakka A,Smidt H,de Vos WM

更新日期：2009-07-01 00:00:00

abstract：:As faecal contamination of recreational and drinking water impairs the water quality and threatens public health, water bodies are routinely monitored for faecal coliforms to detect contamination. However, faecal coliforms are facultative anaerobes that survive and reproduce in ambient waters, and their presence does ...

journal_title：Environmental microbiology

authors： Hong PY,Wu JH,Liu WT

更新日期：2009-07-01 00:00:00

abstract：:Since Robert Koch and colleagues found agar to be an effective gelling agent over a century ago, the pure culture method using agar plates has long been a standard of microbiology. Agar is undoubtedly easy to handle and useful for culture of microorganisms, but recent discovery of the ubiquity of microorganisms that c...

journal_title：Environmental microbiology

authors： Tamaki H,Hanada S,Sekiguchi Y,Tanaka Y,Kamagata Y

更新日期：2009-07-01 00:00:00

abstract：:Commensal bacteria play a role in the aetiology of inflammatory bowel diseases (IBD). High intestinal numbers of Escherichia coli in IBD patients suggest a role of this organism in the initiation or progression of chronic gut inflammation. In addition, some E. coli genotypes are more frequently detected in IBD patient...

journal_title：Environmental microbiology

authors： Wohlgemuth S,Haller D,Blaut M,Loh G

更新日期：2009-06-01 00:00:00

abstract：:The marine alphaproteobacterium Roseovarius nubinhibens ISM can produce the gas dimethyl sulfide (DMS) from dimethylsulfoniopropionate (DMSP), a widespread secondary metabolite that occurs in many phytoplankton. Roseovarius possesses a novel gene, termed dddP, which when cloned, confers on Escherichia coli the ability...

journal_title：Environmental microbiology

authors： Todd JD,Curson AR,Dupont CL,Nicholson P,Johnston AW

更新日期：2009-06-01 00:00:00

abstract：:Marinobacter santoriniensis NKSG1(T) is a mesophilic, dissimilatory arsenate-reducing and arsenite-oxidizing bacterium isolated from an arsenate-reducing enrichment culture. The inoculum was obtained from arsenic-rich shallow marine hydrothermal sediment from Santorini, Greece, with evidence of arsenic redox cycling. ...

journal_title：Environmental microbiology

authors： Handley KM,Héry M,Lloyd JR

更新日期：2009-06-01 00:00:00

abstract：:Bioactive amounts of antibiotics as well as resistant bacteria reach the soil through manure fertilization. We investigated plasmids that may stimulate the environmental spread and interspecies transfer of antibiotic resistance. After treatment of two soils with manure, either with or without the sulfonamide antibioti...

journal_title：Environmental microbiology

authors： Heuer H,Kopmann C,Binh CT,Top EM,Smalla K

更新日期：2009-04-01 00:00:00

abstract：:DNA-SIP (stable isotope probing) was conducted on anaerobic municipal solid waste samples incubated with (13)C-cellulose, (13)C-glucose and (13)C-acetate under mesophilic conditions. A total of 567 full-length bacterial and 448 1100-bp-length archaeal 16S rRNA gene sequences were analysed. In the clone libraries deriv...

journal_title：Environmental microbiology

authors： Li T,Mazéas L,Sghir A,Leblon G,Bouchez T

更新日期：2009-04-01 00:00:00

abstract：:Thermus thermophilus HB27 is well known for its extraordinary trait of high frequencies of natural transformation, which is considered a major mechanism of horizontal gene transfer. We show that the DNA translocator of T. thermophilus binds and transports DNA from members of all three domains. These results, together ...

journal_title：Environmental microbiology

authors： Schwarzenlander C,Haase W,Averhoff B

更新日期：2009-04-01 00:00:00

abstract：:Molecular signatures of new, as yet uncultured mollicute-like organisms (MLOs) have been detected in total rRNA and DNA extracted from tissues, gut contents and casts of four species of the earthworm family Lumbricidae. The MLO 16S rRNA sequences exhibited low identity to those of known Mollicutes species and formed a...

journal_title：Environmental microbiology

authors： Nechitaylo TY,Timmis KN,Golyshin PN

更新日期：2009-04-01 00:00:00

abstract：:A 47 kb genomic island (GEI) bracketed by 50 bp direct repeats, containing 52 annotated genes, was found to delete spontaneously from the genome of Desulfovibrio vulgaris Hildenborough. The island contains genes for site-specific recombinases and transposases, rubredoxin:oxygen oxidoreductase-1 (Roo1) and hybrid clust...

journal_title：Environmental microbiology

authors： Johnston S,Lin S,Lee P,Caffrey SM,Wildschut J,Voordouw JK,da Silva SM,Pereira IA,Voordouw G

更新日期：2009-04-01 00:00:00

abstract：:The gene abundance and gene expression of six diazotroph populations from the Eastern Equatorial Atlantic in June 2007 were examined using nifH gene quantitative polymerase chain reaction (q PCR) methods. Of all the diazotrophs, Trichodesmium spp. was the most abundant with the highest number of gene copies in the Gul...

journal_title：Environmental microbiology

authors： Foster RA,Subramaniam A,Zehr JP

更新日期：2009-04-01 00:00:00

abstract：:This study presents comparative geochemical, microbiological and molecular analyses of Greenland GISP2 ice core samples representing different depths, ages, deposition climates, in situ temperatures, and gas and ionic compositions. Our goal was to determine whether specific organisms, preserved at different depths, co...

journal_title：Environmental microbiology

authors： Miteva V,Teacher C,Sowers T,Brenchley J

更新日期：2009-03-01 00:00:00

abstract：:Marine Synechococcus are frequently found in environments where iron (Fe) is a limiting nutrient. To understand their capacity to respond to Fe stress, we screened picoplankton genomes and the Global Ocean Survey metagenome for known Fe stress genes. Many open ocean strains of Synechococcus lack most known genes for F...

journal_title：Environmental microbiology

authors： Rivers AR,Jakuba RW,Webb EA

更新日期：2009-02-01 00:00:00

abstract：:In diseased piglets from two Dutch pig-breeding farms with neonatal diarrhoea for more than a year, culture and PCR analyses identified the involved microorganism as Clostridium difficile PCR ribotype 078 harbouring toxin A (tcdA) and B (tcdB), and binary toxin genes. Isolated strains showed a 39 bp deletion in the tc...

journal_title：Environmental microbiology

authors： Debast SB,van Leengoed LA,Goorhuis A,Harmanus C,Kuijper EJ,Bergwerff AA

更新日期：2009-02-01 00:00:00

abstract：:The phylogenetic diversity of Bacteria and Archaea within a biodegraded, mesothermic petroleum reservoir in the Schrader Bluff Formation of Alaska was examined by two culture-independent methods based on fosmid and small-subunit rRNA gene PCR clone libraries. Despite the exclusion of certain groups by each method, the...

journal_title：Environmental microbiology

authors： Pham VD,Hnatow LL,Zhang S,Fallon RD,Jackson SC,Tomb JF,DeLong EF,Keeler SJ

更新日期：2009-01-01 00:00:00

abstract：:The factors that control the production of microcystins (hepatotoxins) during cyanobacterial blooms, and the function of these metabolites remain largely unknown. In an attempt to provide answers to these questions, we compared the fitness of microcystin (MC)-producing and non-MC-producing Planktothrix agardhii strain...

journal_title：Environmental microbiology

authors： Briand E,Yéprémian C,Humbert JF,Quiblier C

更新日期：2008-12-01 00:00:00

abstract：:Traditionally, organisms responsible for major biogeochemical cycling processes have been determined by physiological characterization of environmental isolates in laboratory culture. Molecular techniques have, however, confirmed the widespread occurrence of abundant bacterial and archaeal groups with no cultivated re...

journal_title：Environmental microbiology

authors： Prosser JI,Nicol GW

更新日期：2008-11-01 00:00:00

abstract：:Autotrophic ammonia oxidation occurs in acid soils, even though laboratory cultures of isolated ammonia oxidizing bacteria fail to grow below neutral pH. To investigate whether archaea possessing ammonia monooxygenase genes were responsible for autotrophic nitrification in acid soils, the community structure and phylo...

journal_title：Environmental microbiology

authors： Nicol GW,Leininger S,Schleper C,Prosser JI

更新日期：2008-11-01 00:00:00

abstract：:Marine Crenarchaeota represent an abundant component of the oceanic microbiota that play an important role in the global nitrogen cycle. Here we report the association of the colonial ascidian Cystodytes dellechiajei with putative ammonia-oxidizing Crenarchaeota that could actively be involved in nitrification inside ...

journal_title：Environmental microbiology

authors： Martínez-García M,Stief P,Díaz-Valdés M,Wanner G,Ramos-Esplá A,Dubilier N,Antón J

更新日期：2008-11-01 00:00:00

abstract：:We designed and tested a set of specific primers for specific PCR amplification of the biotin carboxylase subunit gene (accC) of the Acetyl CoA carboxylase (ACCase) enzyme. The primer set yielded a PCR product of c. 460 bp that was suitable for denaturing gradient gel electrophoresis (DGGE) fingerprinting followed by ...

journal_title：Environmental microbiology

authors： Auguet JC,Borrego CM,Bañeras L,Casamayor EO

更新日期：2008-10-01 00:00:00

abstract：:The complex ecology of free-living amoebae (FLA) and their role in spreading pathogenic microorganisms through water systems have recently raised considerable interest. In this study, we investigated the presence of FLA and amoebae-resisting bacteria (ARB) at various stages of a drinking water plant fed with river wat...

journal_title：Environmental microbiology

authors： Thomas V,Loret JF,Jousset M,Greub G

更新日期：2008-10-01 00:00:00

abstract：:Substrate supply and protist grazing are two of the most important forces that determine the composition and properties of bacterial assemblages. General ecological theory predicts that the relative importance of these factors is changing with the environmental productivity. In the present study, the interplay between...

journal_title：Environmental microbiology

authors： Corno G,Jürgens K

更新日期：2008-10-01 00:00:00

abstract：:When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa...

journal_title：Environmental microbiology

authors： Barken KB,Pamp SJ,Yang L,Gjermansen M,Bertrand JJ,Klausen M,Givskov M,Whitchurch CB,Engel JN,Tolker-Nielsen T

更新日期：2008-09-01 00:00:00

abstract：:The coral holobiont is the integrated assemblage of the coral animal, its symbiotic algae, protists, fungi and a diverse consortium of Bacteria and Archaea. Corals are a model system for the study of symbiosis, the breakdown of which can result in disease and mortality. Little is known, however, about viruses that inf...

journal_title：Environmental microbiology

authors： Marhaver KL,Edwards RA,Rohwer F

更新日期：2008-09-01 00:00:00

abstract：:The presence of aerobic anoxygenic phototrophs (AAPs) was recently reported from various marine environments; however, there is little information regarding their distribution in fresh waters. We surveyed a number of freshwater systems in central Europe, by infra-red fluorometry, infra-red epifluorescence microscopy, ...

journal_title：Environmental microbiology

authors： Masín M,Nedoma J,Pechar L,Koblízek M

更新日期：2008-08-01 00:00:00

abstract：:The biofilm phenotype of Pseudomonas aeruginosa enables this opportunistic pathogen to develop resistance to the immune system and antimicrobial agents. Pseudomonas aeruginosa biofilms are generated under varying levels of shear stress, depending on the infection site. In the lung mucus of cystic fibrosis (CF) patient...

journal_title：Environmental microbiology

authors： Crabbé A,De Boever P,Van Houdt R,Moors H,Mergeay M,Cornelis P

更新日期：2008-08-01 00:00:00

abstract：:The functioning of natural microbial ecosystems is influenced by various biotic and abiotic conditions. The careful experimental manipulation of environmental conditions can drive microbial ecosystems toward exhibiting desirable types of functionality. Such manipulations can be systematically approached by viewing the...

journal_title：Environmental microbiology

authors： Vandecasteele FP,Crawford RL,Hess TF

更新日期：2008-07-01 00:00:00

abstract：:The contribution of Chloroflexi-type SAR202 cells to total picoplankton and bacterial abundance and uptake of D- and L-aspartic acids (Asp) was determined in the different meso- and bathypelagic water masses of the (sub)tropical Atlantic (from 35 degrees N to 5 degrees S). Fluorescence in situ hybridization (FISH) rev...

journal_title：Environmental microbiology

authors： Varela MM,van Aken HM,Herndl GJ

更新日期：2008-07-01 00:00:00

abstract：:Resting spore formation of some aphid-pathogenic Entomophthorales is important for the seasonal pattern of their prevalence and survival but this process is poorly understood. To explore the possible mechanism involved in the process, Pandora nouryi (obligate aphid pathogen) interacted with green peach aphid Myzus per...

journal_title：Environmental microbiology

authors： Huang ZH,Feng MG

更新日期：2008-07-01 00:00:00

abstract：:In aerobic chemostat cultures maintained at 50% dissolved O(2) tension (3.5 mg l(-1) dissolved O(2)), Shewanella oneidensis strain MR-1 rapidly aggregated upon addition of 0.68 mM CaCl(2) and retained this multicellular phenotype at high dilution rates. Confocal microscopy analysis of the extracellular matrix material...

journal_title：Environmental microbiology

authors： McLean JS,Pinchuk GE,Geydebrekht OV,Bilskis CL,Zakrajsek BA,Hill EA,Saffarini DA,Romine MF,Gorby YA,Fredrickson JK,Beliaev AS

更新日期：2008-07-01 00:00:00

abstract：:The genetic and biochemical basis of defence mechanisms in plant pathogenic fungi against antifungal compounds produced by antagonistic microorganisms is largely unknown. The results of this study show that both degradative and non-degradative defence mechanisms enable the plant pathogenic fungus Botrytis cinerea to r...

journal_title：Environmental microbiology

authors： Schouten A,Maksimova O,Cuesta-Arenas Y,van den Berg G,Raaijmakers JM

更新日期：2008-05-01 00:00:00

abstract：:Magnetotactic bacteria are a heterologous group of motile prokaryotes, ubiquitous in aquatic habitats and cosmopolitan in distribution. Here, we studied the diversity of magnetotactic bacteria in a seawater pond within an intertidal zone at Huiquan Bay in the China Sea. The pond is composed of a permanently submerged ...

journal_title：Environmental microbiology

authors： Pan H,Zhu K,Song T,Yu-Zhang K,Lefèvre C,Xing S,Liu M,Zhao S,Xiao T,Wu LF

更新日期：2008-05-01 00:00:00

abstract：:Certain filamentous cyanobacteria, including Aphanizomenon ovalisporum, are potentially toxic owing to the formation of the hepatotoxin cylindrospermopsin. We previously identified a gene cluster in A. ovalisporum likely to be involved in cylindrospermopsin biosynthesis, including amidinotransferase (aoaA) and polyket...

journal_title：Environmental microbiology

authors： Shalev-Malul G,Lieman-Hurwitz J,Viner-Mozzini Y,Sukenik A,Gaathon A,Lebendiker M,Kaplan A

更新日期：2008-04-01 00:00:00